Lab Simulator
Learn how to operate the FTIR machine by agilent while solving a case of finding bacteria in food
Practice Skills
You've been in the lab many times, now it is time to identify quickly what can go wrong with your samples and readings.
This is the case
A Greasy Spoon restaurant is apparently the source of a salmonella outbreak that has four confirmed victims, two of whom remain hospitalized. Thursday morning, Environmental Health officers held an administrative hearing with the restaurant's owner, Gabino Trujillo Diaz, conducted a follow-up inspection and decided to shut the restaurant down temporarily. Your lab has been supplied with some samples and now your task is to determine the types of bacteria present on the samples.
mhm...I quit!
Ok, lets get started
EN
FR
Learn how the bacteria samples are prepared
EN
FR
Get some help on how to operate the FTIR equipment
Hands on! Get the FTIR equipment ready
How Would You Like to Start?
X EXIT
Close
Close
Close
Close
What Materials are Used in Sample Preparation?
Option 1
Option 2
Option 3
Next
X EXIT
Change objective
Place sample
Move Sample
Adjust Condenser
Focus
Back of equipment
Minimize
Maximize
X EXIT
Fill tank with liquid Nitrogen
Switch camera ON
Front of equipment
PROGRESS LOG
The process of taking a sample measure with a FTIR machine can be tricky, but it simply involves 5 steps that we will layout for you here. In this area we will be providing some instructions to follow and let you manipulate the controls in the machine and computer to comlpete the process.
Ok, thanksAUTOMATIC SAMPLE ANALYSIS
Congratulations! You have obtained your sample reading. Now, it is time to analyze it We are going to use software that interprets the data that you collected. The software is so simple that we will let you figure it out. The name of software is "Bacteria Image" (It is on the desktop of the computer).
X EXIT
Sample Analysis: Completed
Great work! Your sample results are great and we have determined that E coli ATCC 8739 was found to be the cause of this food poisoning incident. That greasy spoon restaurant is going to be shut down for a while.
Now it's Time to Practice Your Skills >
Correct
Notice the difference > 4000, this is indicative of high throughput energy
Incorrect
Notice the difference > 4000, this is indicative of high throughput energy
Is the system well calibrated?
Yes No
Multicolored Map
Energy Values
Optical Image
Correct - The multi colored map is indicative of a non-uniform sample. The optical image shows variability, and the spread of energy values is indicative of a non-uniform sample
Incorrect - The multi colored map is indicative of a non-uniform sample. The optical image shows variability, and the spread of energy values is indicative of a non-uniform sample
Is the energy distribution uniform across the sample?
Yes No
Multicolored Map
Energy Values
Optical Image
Correct - The energy distribution is mostly uniform
Incorrect - The energy distribution is mostly uniform
Is the energy distribution uniform across the sample?
Yes No
(Range 1.3 - 0.1) - 1.3 red - < 0.4 blue
Correct - This is a very thin sample resulting in weak absorbance (notice the scale). The inspection of the optical image shows very uniform sample deposited, and the uniform blue image confirms the sample is uniform. However, the color blue means that the absorbance is not optimum. Blue here indicates too little intensity, meaning not enough sample.m
Incorrect - This is a very thin sample resulting in weak absorbance (notice the scale). The inspection of the optical image shows very uniform sample deposited, and the uniform blue image confirms the sample is uniform. However, the color blue means that the absorbance is not optimum. Blue here indicates too little intensity, meaning not enough sample.
Is the sample too thin?
Yes No
(Range 1.3 - 0.1) - 1.3 red - < 0.4 blue
Correct - TUniform sample (see optical image), the single color (blue) demonstrates uniformity. The absorbance, Y-scale at 0.08, shows that sample is too thin.
Incorrect - Uniform sample (see optical image), the single color (blue) demonstrates uniformity. The absorbance, Y-scale at 0.08, shows that sample is too thin.
Is the sample too thin?
Yes No
(Range 1.3 - 0.1) - 1.3 red - < 0.4 blue
Correct - Uniform sample (see optical image), the single color (blue) demonstrates uniformity. The absorbance, Y-scale at 0.4, shows sample is too thin.
Incorrect - Uniform sample (see optical image), the single color (blue) demonstrates uniformity. The absorbance, Y-scale at 0.4, shows sample is too thin.
Is the sample too thin?
Yes No
(Range 1.3 - 0.1) - 1.3 red - < 0.4 blue
Correct - Uniform sample uniform (see optical image), the single color (blue) demonstrates uniformity. The absorbance, Y-scale at 0.3, shows sample is too thin.
Incorrect - Uniform sample uniform (see optical image), the single color (blue) demonstrates uniformity. The absorbance, Y-scale at 0.3, shows sample is too thin.
Is the sample too thin?
Yes No
Correct - Uniform sample (see optical image), the single color (blue) demonstrates uniformity. The absorbance, Y-scale at 2.0, shows sample is too thick.
Incorrect - Uniform sample (see optical image), the single color (blue) demonstrates uniformity. The absorbance, Y-scale at 2.0, shows sample is too thick.
Is the sample too thick?
Yes No
Correct - The optical image shows a defined film of bacteria. In the image the red section has absorbance > 1.3, this is not usable.
Incorrect - The optical image shows a defined film of bacteria. In the image the red section has absorbance > 1.3, this is not usable.
Is the sample too thick?
Yes No
The sample thickness is not uniform (see optional image). The Dark red spots are unusable, the orange-green areas are usable, and the blue spots are unusable.
The sample thickness is not uniform (see optional image). The Dark red spots are unusable, the orange-green areas are usable, and the blue spots are unusable.
Is the sample too thick?
Yes No
Correct - In fact this is a good sample. The optical image shows uniformity, the color image also shows uniformity, and the pixels are mostly orange and green.
Incorrect - In fact this is a good sample. The optical image shows uniformity, the color image also shows uniformity, and the pixels are mostly orange and green.
Is the sample too thick?
Yes No